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分類清單
謝家慶
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謝家慶 副教授

Dr. Jia-Ching Shieh


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Tel:(04)2473-0022 ext 11806; 12371

Fax:(04)2324-8187

jcs@csmu.edu.tw

學經歷
學歷:
  • 英國曼徹斯特大學生化暨分子生物研究所博士
經歷:
  1. 財團法人生物技術開發中心 研發部蛋白質化學組 副組長暨研究員2001/09至2003/02
  2. Laboratory of molecrlar biology Division of Structure Studies Staff Scientist 1998/12至2001/08
  3. National Institute for Medical Research Division of Yeast Genetics Non-clinical Scientist 1995/01至1998/11
  4. Div. of Biochem.& Mole. Biol., University of Manchester, Research Assistant 1992/02至1994/08
現任:
  • 生醫系副教授

研究方向及計畫
  • 2012/8-2015/12 釐清白色念珠菌CDC4在型態生成及逆境反應之對話所扮演的角色(國科會-科技部)
  • 2009/01-2011/12 以蛋白質學鑑定調節白色念珠菌型態形成之主要成分(國衛院)
  • 2008/08-2011/7 解析白色念珠菌CaCDC7在型態形成上的新穎功能(國科會)
  • 2003/08-2004/07 以噬菌體基因庫選質對酵母菌基因啟動仔專一性的鋅指蛋白建構重組轉錄音子來探討對影響基因表現和外表型的影響(基因體計畫)
  • 2002/06-2003/05 肝醣貯積症第三型酵素治療之有效性確認(基因體計畫)
  • 2001/09-2003/12 Tailor-made transcription factors directing the control of gene expression(英國MRC)

專長及教授課程
  • 形態形成
  • 分子遺傳學
  • 功能基因體學
  • 單細胞真核模式生物
  • 細胞週期及訊息調控
研究著作
  1. 期刊論文
    1. Chien T, Tseng TL, Wang JY, Shen YT, Lin TH, Shieh JC (2015) Candida albicans DBF4 gene inducibly duplicated by the mini-Ura-blaster is involved in hypha-suppression. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 779:78-85.

    2. Tseng TL, Lai WC, Lee TL, Hsu WH, Sun YW, Li WC, Cheng CW, Shieh JC (2015) A role of Candida albicans CDC4 in the negative regulation of biofilm formation. Canadian Journal of Microbiology 61(4):247-55.

    3. Chin C, Lai WC, Lee TL, Tseng TL, Shieh JC (2013) Dissection of the Candida albicans Cdc4 protein reveals the involvement of domains in morphogenesis and cell flocculation. Journal of Biomedical Science, 20(1):97, 1-11.

    4. Shyu HY, Shieh JC, Lin JH, Wang HW, and Cheng CW (2012) Polymorphisms of DNA repair pathway genes and cigarette smoking in relation to susceptibility of large artery atherosclerotic stroke among ethnic Chinese in Taiwan. Journal of Atherosclerosis and Thrombosis. 19(4):316-25.

    5. Lai WC, Tseng TL, Jian T, Lee TL, Cheng CW, Shieh JC (2011) Construction of Candida albicans Tet-on tagging vectors with a Ura-blaster cassette. Yeast, 28(3), 253-263.

    6. Tseng,T-L., Lai, W-C., Jian, T., Li, C., Sun, Sunny H-F., Way, T-D., and Shieh, J-C. (2010) Affinity purification of Candida albicans CaCdc4 associated proteins reveals the presence of novel proteins involved in morphogenesis. Biochem Biophs Commun; 2010 April 23; 395(1):152-57. Epubl 2010 Mar 31. (SCI)

    7. Jia-Ching Shieh (2010) Chapter 3 Combinatorial Design: Bipartite selection of zinc fingers by phage display for any 9-bp DNA target site. Engineered Zinc Finger Proteins: Methods and Protocols (Methods in Molecular Biology) (ISBN: 1607617528; ISBN-13:9781607617525 ). New York: Humana Press.

    8. Shyu,, H-Y., Fong, C-S, Fu, Y-P., Shieh, J-C., Yin J-H., Chang, C-Y., Wang, H-W., Cheng, C-W. (2010) Genotype polymorphisms of GGCX, NQO1, and VKORC1 genes associated with risk susceptibility in patients with large-artery atherosclerotic strok. Clinica Chimica Acta; 2010 Jun3; 11(11-12):840-45. Epubl 2010 Mar 1. (SCI)

    9. Hong, H-M., Yang, J-J., Shieh, J-C., Li, M-L., and Li, S-Y. (2010). Novel mutations in the connexin43 (GJA1) and rGJA1 pseudogene may contribute to nonsyndromic hearing loss Human Genetics; 2010 Mar; 127(5):545-51. Epubl 2010 Feb 4. (SCI)

    10. Lin, J-N., Lin, C-H., Rau, K-M., Shieh, P-C., Kuo, D-H., Shieh, J-C., Chen, W-J., Tsai, S-C., and Way, T-D. (2010) Resveratrol modulates tumor cell proliferation and protein translation via SIRT1-dependent AMPK activation. J Agr Food Chem; 2010 Feb 10; 58(3):1584-41. (SCI)

    11. Cheng, C-W., Yu, J-C., Wang, H-W., Huang, C-S., Shieh, J-C., Fu, Y-P., Chang, C-W., Wu, P-E., and Shen, C-Y. (2010). The clinical implications of MMP-11 and CK-20 expression in human breast cancer. Clinica Chimica Acta; 2010 Feb; 411(3-4):234-41. (SCI)

    12. Way, T-D, Lin, H-Y., Kuo, D-H., Tsai, S-J., Shieh, J-C., Wu, J-C., Lee, M-R., and Lin, J-K. (2009) Pu-erh Tea Attenuates Hyperlipogenesis and Induces Hepato ma Cells Growth Arrest through Activating AMPK in Hu ma n HepG2 Cells. J Agr Food Chem (57(12): 5257-64 (SCI)

    13. Cheng, C-W., Yu, J-C., Huang, C-S., Shieh, J-C., Fu., Y-O., Wang, H-W. and Shen, C-Y (2008) Polymorphism of cytosolic serine hydroxymethyltransferase, estrogen and breast cancer risk among Chinese women in . Breast Cancer Res. Treat. 111 (1): 145-155 (SCI)

    14. Shieh, J-C., Cheng, Y-J., Su, M-C., Isalan, M., Moore , M., Choo, Y. and Klug, A. (2007) Tailor- ma de zinc finger-transcription factors activate the expression of FLO11 gene with phenotypic consequence in the yeast Saccharomyces cerevisiae. PLoS ONE 2(8): e746:1-10. (SCI)

    15. Fong, C-S., Wu, R-M., Shieh, J-C., Chao, Y-T., Kuao, C-L., and Cheng, C-W (2007). Effect of Pesticide Exposure on Genetic Factors Associated with Parkinson’s Disease in Southwestern Taiwanese. Clin Chim Acta 378(1-2): 136-141. (SCI)

    16. Su, C-C., Yang, J-J., Shieh, J-C., Su, M-C., Li, S-Y (2006). Identification of novel mutations in the KCNQ4 gene of patients with nonsyndromic deafness from . Audiol Neurotol 12(1): 20-26. (SCI)

    17. Shieh, J-C., White, A., Y-C., Cheng and Rosamond, J. (2005) Identification and functional characterization of Candida albicans CDC4. J Biomed Sci. 12(6): 913-924. (SCI)
  2. 研討會論文 (2010-2015)
    • Shieh JC, Jian T, Tseng TL, Wang JY, Shen YT, Lee TL, Cheng CW, Chuan Li (2015, Aug.). Candida albicans Dbf4-dependent kinase plays a novel role in suppression of yeast-to-hypha transition. BIT's 5th Annual World Congress of Microbes 2015. Shanghai, China. NSC 101-2629-B-040-001-MY3.

    • Lai WC, Sun HFS, Lin H, Shieh JC (2015, May). Tetracycline-regulated Candida Albicans Pho85p Expression in Responses of Environmental Changes. International Conference on Chemical, Biology and Environmental Sciences (ICCBES), Kyoto, Japan. NSC 101-2629-B-040-001-MY3.

    • Lai WC, Sun HFS, Lin PH, Lin H, Shieh JC (2015, Mar). Study of the GCN4-THR1 Dependent Nutrient Response in Candida albicans TOR Signaling. 30th Joint Annual Conference of Biomedical Science, National Defense Medical Center, Taipei, Taiwan. NSC 101-2629-B-040-001-MY3.

    • Lai WC, Sun HFS, Lin PH, Lin H, Shieh JC (2015, Mar). Study of the GCN4-THR1 Dependent Nutrient Response in Candida albicans TOR Signaling. 30th Joint Annual Conference of Biomedical Science, National Defense Medical Center, Taipei, Taiwan. NSC 101-2629-B-040-001-MY3.

    • Lai WC, Lin PH, Shieh JC (2014, Mar). Development of a Dominant Selection System to Create Cagcn4 Null Mutants for the Assessment of Nutrient Sensing in Candida albicans. 29th Joint Annual Conference of Biomedical Science, National Defense Medical Center, Taipei, Taiwan. NSC 101-2629-B-040-001-MY3.

    • Lin YP, Hsu WH, Lai WC, Shieh JC (2014, Mar). Assessment of Candida albicans H3H4 Demethylase-encoding Gene JHD2 on Virulence-related Characteristics. 29th Joint Annual Conference of Biomedical Science, National Defense Medical Center, Taipei, Taiwan. NSC 101-2629-B-040-001-MY3.

    • Jia-Ching Shieh (2013, Aug). Candida albicans DBF4 Gene is inducibly Duplicated by the Mini-Ura Blaster and Involved in a Novel Role of Hyphae-Suppression. The 3rd Annual International Symposia of Mycology (ISM-2013) under BIT's 3rd Annual World Congress of Microbes-2013, Shangri-La Hotel, Wuhan, China. NSC 97-2320-B-040-014-MY3. Invited Speaker and Session Chair

    • Lai WC, Sun HFS, Shieh JC (2013, Mar). Development of a Tet-on Based BiFC System to Explore Protein-protein Interaction in Candida albicans. 3rd Annual International Conference on Advances on Biotechnology (BIOTECH 2013), Singapore. Lai WC had been awarded one of the two best student papers. http://www.advbiotech.org/.

    • Chin C, Lai WC, Lee TL, Tseng TL, Shieh JC (2012, Dec). Dissection of the Candida albicans Cdc4 protein reveals its essential domains and the differentially regulated regions involved in morphogenesis cell flocculation. The 23rd International Conference on Genome Informatics, Tainan, Taiwan.

    • Hsu WH, Shieh JC, Tseng TL, Lai WC (2012, Mar). Dissecting the role of CaCdc4-associated protein CaJhd2, a putative H3 lysine 4 demethylase. 27th Joint Annual Conference of Biomedical Science, Taipei, Taiwan. NSC 99-2815-C-040-013-B

    • Li WC, Shieh JC, Tseng TL, Jian T, Lai WC (2012, Mar). Study of the functional interaction between Candida albicans GPH1 and CDC4 on morphogenesis and stress response. 27th Joint Annual Conference of Biomedical Science, Taipei, Taiwan. NSC 100-2815-C-040-028-B.

    • Lai WC, Tseng TL, Jian T, Lee TL, Cheng CW, Shieh JC (2011, Aug). Construction of Candida albicans Tet-on tagging vectors with a Ura-blaster cassette. 2011 Symposium on Frontiers of Biomedical Sciences, China Medical University, Taichung, Taiwan.

    • Yan, SY, Wang JY, Jian T, Shieh JC (2010, Mar). Candida albicans Dbf4 and Cdc7 are functionally dependent for suppression of yeast-to-hyphae transition. 25th Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan.

    • Shieh J-C. Tschen-wei Chang, T-w.,Chang, H.W., Jian, T., Tseng, T-L., and Cheng, Y-C. A novel role of Candida albicans CDC7 in morphogenesis. International Conference on Yeast Genetics and Molecular Biology. August 19th – 24th, Manchester, (Poster 170) Yeast 2009; 26:S1.

    • Jian, T., Tseng, T-L., and Shieh, J-C. (2009) Elucidation the function of DDK regulatory subunit DBF4 in Candida albicans. 24th Joint Annual Conference of Biomedical Science (Poster P370, p226)

    • Shieh, J-C. (2008) Stress-activated protein kinase pathway in the fission yeast Schizosaccharomyces pombe. 7th Annual Yeast Meeting, National Taiwan University , College of Medicine , September 1st, 2008 (Oral presentation)

    • Lai, W-C.*, Shieh, J-C. (2008) Dissecting the function of Candidal albicans SCFCDC4. 23rd Joint Annual Conference of Biomedical Sciences (Oral presentation O29, p74).

    • Chang, T-W., Wu, C-H., and Shieh, J-C. (2008) Candida albicans CDC7 has a novel role in morphogenesis. 23rd Joint Annual Conference of Biomedical Sciences (Poster P152, p155).

    • Tseng, T-L., Lai, W-C., and Shieh, J-C. (2008) Affinity purification of the Candida albicans Cdc4 associated proteins. 23rd Joint Annual Conference of Biomedical Sciences (Poster P179, p164).

    • Shieh, J-C. (2007) Tailor- ma de transcription factors with zinc finger DNA-binding modules for sequence-specificity activate gene expression with phenotypic consequences in the yeast Saccharomyces cerevisiae. International Conference on Yeast Genetics and Molecular Biology. July 1st – 6th, Melbourne, (Poster 08-31, S87) Yeast 2007; 24:S1-S175. Oral and Poster presentation.

    • Shieh, J-C. (2007) Yeast-filament Transition in Candida albicans Mediated by SCF Ubiquitin Ligases and Beyond. 2007 Symposium of Medical Mycology. 8 July 2007 . Invited speaker.

    • Chang, M-W., Lai, W-C., Tseng, T-L., Ting, J., and Shieh, J-C. (2007) SCF ubiquitin ligases mediate morphological transition in Candida albicans. 22nd Joint Annual Conference of Biomedical Sciences.

    • Cheng,Y-C. and Shieh, J-C. (2006) Candida albicans SCFCDC4 is a negative regulator of filamentous growth. 21st Joint Annual Conference of Biomedical Sciences.

    • Shieh, J-C., Choo, Y., Klug, A, et al. (2005) Tailor- ma de transcription factors on C2H2 zinc finger module are used as gene switches to direct gene expression in Saccharomyces cerevisiae. 13th Symposium on Recent Advances in Cellular and Molecular Biology (Poster No.8, P. 062).

    • Chu , P-H., Chen, Y-C., Liu, C-C., Shieh, J-C., and Lin, B-L. (2004) Improvement of the Cardiac Function by Gene Therapy. Asian Federation of Societies for Ultrasound in Medicine (AFSUMB); (#10013).

    • Chen, Y-C., Wu, H-H., Shiu, T-F., Liu, C-C., Shieh, J-C., Lin, B-L., and Chu , P-H. (2004) Improvement of the mortality and morbidity using gene therapy by direct injection into left ventricular cardiomyocytes. Chinese Society of Laboratory Ani ma l Sciences (Dec. 2nd –Dec. 3rd), Taipei, .

    • Chu , P-H., Chen, Y-C., Liu, C-C., Shien, J.C., and Lin, B-L. (2003) Improvement of the Cardiac Function Using Gene Therapy by Direct Injection in Left Ventricle: Following up by Echocardiography. Acta Cardio Sinica ;19S:037, Taipei

    • Liu, C-C, Cheng, H-C., Chen, J-C., Shieh, J-C., and Lin, B-L. (2002) Preparation of adeno-associated virus and adenovirus vectors. The 2nd International Symposium on DNA Vaccine and Gene Therapy Technology p30.

    • Shieh, J-C., Choo, Y., Isalan, M., Moore , M., Choo, K-B., Hong, S-Y. and Klug, A. (2002) Engineering zinc finger proteins to target at promoters of endogenous genes for regulating their expression in yeast. The 4th Asian-Pacific Organization for Cell Biology Congress-Cell Biology in the Post-Genomic Era (MS16-2, oral presentation)
  3. 技術報告
    • Chen, Y-C. and Shieh, J-C. (2002) In vivo gene transfer through left ventrical injection with short time occlusion of aortic artery and pulmonary artery in cardiomyopathic animal models. 91-EC-2-A-17- 0355:DCB-0335-T230 (91). Development Center for Biotechnology, Taipei, Taiwan, R.O.C.