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Chuan Li (Professor, Ph. D.)
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Chuan Li

(Professor; Ph. D)


Phone

Tel:886-4-2473-0022 ext 12321

E-mail

cli@csmu.edu.tw

Interests in Research

My laboratory has been studying stable protein methylation since 1997 for mostly arginine methylation in lymphoblastoid cells and HeLa cells. We successfully utilized bioinformatic as well as proteomic tools to globally study protein arginine methylation both for the protein arginine methyltransferases (PRMT) and the methylaccepting proteins. Besides, we have been involved in studies of human genetic diseases such as fragile X mental retardation, spinocerebellar ataxia, dentatorubral and pallidoluysian atrophy and Hirschsprung disease.

We also use zebrafish as a model system to study protein arginine methylation We have successfully established the zebrafish culture system and analyzed the expression patterns of the zebrafish PRMT genes and knock-downed the expression of PRMT1 gene by antisense morpholino oligonucleotide (AMO). We have characterized the phenotypes of the morphants and are analyzing the arginine-methylated proteins in embryogenesis.

In our previous proteomic studies, we have identified a few RNA binding proteins that are candidate protein arginine methylated protein. We have obtained evidences of the modification and would like to further pursue the physiological roles of the modification. We thus would like to use cultured human cells as well as zebrafish as the model systems to characterize protein arginine methylation of specific RNA binding proteins and to investigate the involvement of the posttranslational modification in cellular function and embryogenesis.

Publications (at most 7 publications in recent 5 years)

  • Hung, CJ, Chen, DH, Shen, YT , Li, YC, Lin, YW, Hsieh, M and Li, C. (2007) Characterization of Protein Arginine Methyltransferases in Porcine Brain. J. Biochem.and Mol Biol. 40, 617-624. (IF=2.141; 157/263=59.7%)

  • Wang, YC, Wang, ST, Chou, MC, Li, C, Chen, LY, Liu, WH, Chen, PR and Liu, TC. (2008) The Role of T148 and R281 in human dihydrolipoamide dehydrogenase J BioMedical Science 15, 37-46. (IF=2.024; 36/81=44.4%)

  • Hung CJ, Lee YJ, Chen DH, Li C.(2009) Proteomic Analysis of Methylarginine-Containing Proteins in HeLa Cells by Two-Dimensional Gel Electrophoresis and Immunoblotting with a Methylarginine-Specific Antibody. Protein J. 28, 139-147. (IF: 1.010; 231/263)

  • Wu TT, Tsai, TW, Chang H, Su, CC, Li, SY, Lai HS, and Li, C. (2010) Polymorphisms of the RET Genes in Hirschsprung Disease, Anorectal Malformation and Intestinal Pseudo-obstruction in . J. Formos. Med. Assoc. 109, 32-38. (IF; 0.661; 93/132)

  • Tseng TL, Lai WC, Jian T, Li C, Sun HF, Way TD, and Shieh, JC. (2010) Affinity purification of Candida albicans CaCdc4-associated proteins reveals the presence of novel proteins involved in morphogenesis. Biochem. Biophys. Res. Commun. 395, 152-157. (IF:2.548, 146/283)

  • Wu TT, Tsai, TW, Shen, YT, Hsu, JD, Yang, LC and Li, C. (2010) Expression of PRMT1 proteins in human colon tissues from Hirschsprung disease patients. Neurogastroenterology and Motility (IF: 3.568; 15/65)

  • Tsai, Yun-Rong, Pan, Huichin, Hung, Chuan-Mao, Hou, Po-Tsun, Li, Yi-jen, Shen, Yi-Ting, Wu, Trang-Tiau and Li, C. (2011) The predominant protein arginine methyltransferase PRMT1 is critical for zebrafish convergence and extension during gastrulation. FEBS J. 278, 905-917. (IF: 3.042, 115/283)