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Jia-Ching Shieh
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Jia-Ching Shieh

(Associate Professor; Ph. D)


Phone

Tel(Office):886-4-2473-0022 ext 12371
Tel(Lab):886-4-2473-0022 ext 11807

E-mail

jcs@csmu.edu.edu

Interests in Research

Molecular mechanism underlying morphological plasticity of the opportunistic human fungal pathogen Candida albicans: significance in biology and medicine

One of the key features of the opportunistic human pathogenic yeast Candida albicans to infect host and shift from commensally to pathogenic form is its ability in morphogenesis of yeast-to-hypha transition. We have been interested to study CDC4 and CDC7 genes as they are conserved across evolutionary spectrum in G1-to-S phase transition and initiation of DNA replication in the mitotic cell cycle, respectively, and are the vital in coordination of morphogenesis. Unexpectedly, both genes have been found to suppress yeast-to-hypha transition in C. albicans. CDC4-related genes involved in aspects of stress and nutrient responses as well as epigenetic regulation have been identified. The cross-talk between morphogenesis and stress and nutrient responses and the role of epigenetic regulation in morphological plasticity are under investigation using classical approaches of genetics, molecular biology, cell biology and biochemistry incorporated with cutting edge technologies of functional genomics, proteomics, and epigenomics. To overcome C. albicans having a non-canonical codon usage and being a diploid without a complete sexual cycle, novel and non-genetic platforms have been established to reinforce revealing unique genes involved in morphogenesis and other virulence-related factors.
 

Publications (at most 7 publications in recent 5 years)

  1. Lai WC, Chang Tw,Wu CH, Yang SY, Lee TL, Li WC, Chien T, Cheng YC, Shieh JC* (2016, Sep). Candida albicans Dbf4-dependent Cdc7 kinase plays a novel role in the inhibition of hyphal development. Scientific Reports, 6, 33716:1-13. (SCI, 7/63, MULTIDISCIPLINARY SCIENCES ). MOST 105-2320-B-040-027-MY3.

  2. Lai WC, Sun HFS, Lin PH, Lin H, Shieh JC* (2016, Feb). A new rapid and efficient system with dominant selection developed to inactivate and conditionally express genes in Candida albicans. Current Genetics, 62:213-235. (SCI, 76/16, GENETICS & HEREDITY). MOST 101-2629-B-040-001-MY3.

  3. Shieh JC* (2015, Nov.). The conservation of genotoxic stress-induced morphological changes in yeasts. Edorium Journal of Biomedical Science 1:1-6. The Editorial Writing (Non-SCI). MOST 101-2629-B-040-001-MY3. NSC 97-2320-B-040-014-MY3. National Health Research Institutes: NHRI-EX99-9808SI & NHRI-EX100-9808SI.

  4. Chien T, Tseng TL, Wang JY, Shen YT, Lin TH, Shieh JC* (2015, Jun). Candida albicans DBF4 gene inducibly duplicated by the mini-Ura-blaster is involved in hypha-suppression. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 779, 78-85. (SCI, 14/87, TOXICOLOGY). NSC 97-2320-B-040-014-MY3.

  5. Tseng TL, Lai WC, Lee TL, Hsu WH, Sun YW, Li WC, Cheng CW, Shieh JC* (2015, Apr). A role of Candida albicans CDC4 in the negative regulation of biofilm formation. Canadian Journal of Microbiology, 61: 247–255. (SCI, 121/162, BIOTECHNOLOGY & APPLIED MICROBIOLOGY). MOST 101-2629-B-040-001-MY3.

  6. Chin C, Lai WC, Lee TL, Tseng TL, Shieh JC* (2013, Dec). Dissection of the Candida albicans Cdc4 protein reveals the involvement of domains in morphogenesis and cell flocculation. Journal of Biomedical Science, 20(1):97, 1-11. (SCI, 50/123, MEDICINE, RESEARCH & EXPERIMENTAL ). National Health Research Institutes: NHRI-EX100-9808SI.

  7. Liu SY, Huang CH, Shieh JC#, Lee TL (2017, July). Cinnamomum osmophloeum Kanehira ethanol extracts prevents human liver-derived HepG2 cell death from oxidation stress by induction of ghrelin gene expression. Journal of Biosciences, 42(3), 439-448. (SCI, 47/84, BIOLOGY ).