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Jia-Ching Shieh (Associate Professor, Ph. D.)
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Jia-Ching Shieh

(Associate Professor; Ph. D)


Phone

Tel(Office):886-4-2473-0022 ext 12371
Tel(Lab):886-4-2473-0022 ext 11807

E-mail

jcs@csmu.edu.edu

Interests in Research

Molecular mechanism underlying morphological plasticity of the opportunistic human fungal pathogen Candida albicans: significance in biology and medicine

One of the key features of the opportunistic human pathogenic yeast Candida albicans to infect host and shift from commensally to pathogenic form is its ability in morphogenesis of yeast-to-hypha transition. We have been interested to study CDC4 and CDC7 genes as they are conserved across evolutionary spectrum in G1-to-S phase transition and initiation of DNA replication in the mitotic cell cycle, respectively, and are the vital in coordination of morphogenesis. Unexpectedly, both genes have been found to suppress yeast-to-hypha transition in C. albicans. CDC4-related genes involved in aspects of stress and nutrient responses as well as epigenetic regulation have been identified. The cross-talk between morphogenesis and stress and nutrient responses and the role of epigenetic regulation in morphological plasticity are under investigation using classical approaches of genetics, molecular biology, cell biology and biochemistry incorporated with cutting edge technologies of functional genomics, proteomics, and epigenomics. To overcome C. albicans having a non-canonical codon usage and being a diploid without a complete sexual cycle, novel and non-genetic platforms have been established to reinforce revealing unique genes involved in morphogenesis and other virulence-related factors.

Publications (at most 7 publications in recent 5 years)

  • Jia-Ching Shieh (2010) Chapter 3 Combinatorial Design: Bipartite selection of zinc fingers by phage display for any 9-bp DNA target site. Engineered Zinc Finger Proteins: Methods and Protocols (Methods in Molecular Biology) (ISBN: 1607617528; ISBN-13:9781607617525 ). New York: Humana Press.

  • Tseng TL, Lai WC, Jian T, Li C. Sun HF, Way TD, Shieh JC (2010) Affinity purification of Candida albicans CaCdc4-associated proteins reveals the presence of novel proteins invovled in morphogenesis. Biochemical and Biophysical Research Communications, 395(1), 152-157.

  • Lai WC, Tseng TL, Jian T, Lee TL, Cheng CW, Shieh JC (2011) Construction of Candida albicans Tet-on tagging vectors with a Ura-blaster cassette. Yeast, 28(3), 253-263.

  • Shyu HY, Shieh JC, Lin JH, Wang HW, and Cheng CW (2012) Polymorphisms of DNA repair pathway genes and cigarette smoking in relation to susceptibility of large artery atherosclerotic stroke among ethnic Chinese in Taiwan. Journal of Atherosclerosis and Thrombosis. 19(4):316-25.

  • Chin C, Lai WC, Lee TL, Tseng TL, Shieh JC (2013) Dissection of the Candida albicans Cdc4 protein reveals the involvement of domains in morphogenesis and cell flocculation. Journal of Biomedical Science, 20(1):97, 1-11.

  • Tseng TL, Lai WC, Lee TL, Hsu WH, Sun YW, Li WC, Cheng CW, Shieh JC (2015) A role of Candida albicans CDC4 in the negative regulation of biofilm formation. Canadian Journal of Microbiology 61(4):247-55.

  • Chien T, Tseng TL, Wang JY, Shen YT, Lin TH, Shieh JC (2015) Candida albicans DBF4 gene inducibly duplicated by the mini-Ura-blaster is involved in hypha-suppression. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 779:78-85.