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分類清單
李娟
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李娟 教授


Dr. Chuan Li


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Tel:(04)2473-0022 ext 11807;12321

Fax:(04)2324-8187

cli@csmu.edu.tw

學經歷
學歷:
  • 1987 台大農化系 學士
  • 1993 加州大學洛杉磯分校 生化博士
經歷:
  • 2004-present 中山醫學大學生物醫學科學學系 教授
  • 2002-2004 中山醫學大學生物醫學科學學系 系主任
  • 1993-2003 中山醫學院生命科學系 副教授

研究方向及計畫
  • 易脆 X 染色體症候群蛋白 FMRP 轉譯後調控研究(NSC 87-2314-b-040-022)
  • RGG 蛋白之精氨酸甲基化研究 (1/3) (NSC 89-2316-B-040-003-)
  • RGG 蛋白之精氨酸甲基化研究 (2/3) (NSC 89-2320-B-040-072-)
  • RGG 蛋白之精氨酸甲基化研究 (3/3) (NSC 90-2320-B-040-031)
  • 蛋白精氨酸甲基化之蛋白體研究 (1/3) (NSC 91-3112-B-040-001)
  • 提昇大學競爭力重點發展計畫子計畫B大學科技系所人才培育計劃 教育部(91/8/1-92/7/31)
  • 蛋白精氨酸甲基化之蛋白體研究 (2/3) (NSC 92-3112-B-040-001)
  • 大學科技系所人才培育計劃 教育部 (92/8/1-93/7/31)
  • 蛋白精氨酸甲基化之蛋白體研究 (3/3) (NSC 93-3112-B-040-001)
  • 自體免疫疾病中蛋白質精胺酸甲基化之蛋白質體研究(1/3) (NSC93-2745-B-040-002-URD)
  • 提昇私校研發能量專案計畫-自體免疫疾病中心之建立子計劃一:自體免疫疾病中蛋白質精氨酸甲基化之蛋白質體研究(NSC94-2745-B-006-URD)
  • 利用斑馬魚為模式生物系統研究蛋白質精胺酸甲基轉氨酶基因家族(NSC94-2320-B-040-044)
  • 提昇私校研發能量專案計畫-自體免疫疾病中心之建立子計劃一:自體免疫疾病中蛋白質精氨酸甲基化之蛋白質體研究(NSC95-2745-B-040-006-URD)
  • 利用斑馬魚為模式生物系統研究蛋白質精胺酸甲基化(NSC95-2320-B-040-042)
  • 以斑馬魚為模式系統研究蛋白質精胺酸甲基化(1/2) (NSC96-2320-B-040-022-MY2)
  • 以斑馬魚為模式系統研究蛋白質精胺酸甲基化(2/2) (NSC96-2320-B-040-022-MY2)
  • 特定RNA結合蛋白之蛋白質精胺酸甲基化灣此轉譯後修釋在細胞功能及胚胎發育中的參與(1/2)(NSC98-2320-B-040-011-MY3)

專長及教授課程
  • 蛋白化學
  • 分子遺傳學
  • 普通生物學
  • 老化分子機制
研究著作
  1. 期刊論文
    1. Wu TT, Tsai, TW, Shen, YT, Hsu, JD, Yang, LC and Li, C. (2010) Expression of PRMT1 proteins in human colon tissues from Hirschsprung disease patients. Neurogastroenterology and Motility In press

    2. Wu TT, Tsai, TW, Chang H, Su, CC, Li, SY, Lai HS, and Li, C. (2010) Polymorphisms of the RET Genes in Hirschsprung Disease, Anorectal Malformation and Intestinal Pseudo-obstruction in . J. Formos. Med. Assoc. 109, 32-38.

    3. Wu TT, Tsai, TW, Chang H, Su, CC, Li, SY, Lai HS, and Li, C. (2009) Polymorphisms of the RET Genes in Hirschsprung Disease, Anorectal Malfor ma tion and Intestinal Pseudo-obstruction in . J. Formos. Med. Assoc. 108, in press

    4. Hung CJ, Lee YJ, Chen DH, Li C.(2009) Proteomic An aly sis of Methylarginine-Containing Proteins in HeLa Cells by Two-Dimensional Gel Electrophoresis and Immunoblotting with a Methylarginine-Specific Antibody. Protein J. 28, 139-147.

    5. Wang, YC, Wang, ST, Chou, MC, Li, C, Chen, LY, Liu, WH, Chen, PR and Liu, TC. (2008) The Role of T148 and R281 in hu ma n dihydrolipoamide dehydrogenase J BioMedical Science 15, 37-46.

    6. Hung, CJ, Chen, DH, Shen, YT , Li, YC, Lin, YW, Hsieh, M and Li, C. (2007) Characterization of Protein Arginine Methyltransferases in Porcine Brain. J. Biochem.and Mol Biol. 40, 617-624.

    7. Wang YC,Wang ST, Li C, Liu WH,Chen PR,Chen LY, Liu TC. (2007)The Role of N286 and D320 in The Reaction Mechanism of Hu ma n Dihydrolipoamide Dehydrogenase (E3) Center Do ma in. J. BioMedical Science 14, 203-10.

    8. Chang WH, Ce ma l CK, Hsu YH, Kuo CL, Nukina N, Chang MH, Hu HT, Li C, Hsieh M. (2005) Dynamic expression of Hsp27 in the presence of mutant ataxin-3. Biochem Biophys Res Commun. 336, 258-67.

    9. Tsai HF, Lin SJ, Li C, Hsieh M. (2005) Decreased expression of Hsp27 and Hsp70 in transformed lymphoblastoid cells from patients with spinocerebellar ataxia type 7. Biochem Biophys Res Commun. 334, 1279-86.

    10. Wu, T-T, Tsai, T-W, Chu, C-T, Lee, Z-F, Hung, C-M, Su, C-C, Li, S-Y, Hsieh, M, and Li, C. (2005) Low RET Mutation Frequency and Polymorphism An aly sis of the RET and EDNRB gene in patients with Hirschsprung’s disease in Taiwan J Hu ma n Genetics 50,168-74.

    11. Hung, CM and Li, C. (2004) Identification and phylogenetic an aly ses of the protein arginine methyltransferase gene family in fish and ascidians. GENE. 340, 179-187.

    12. Chen, D-H, Wu, KT, Hung, CJ, Hsieh, M, and Li C. (2004) Effects of Adenosine Dialdehyde Treatment on in vitro and in vivo Stable Protein Methylation in HeLa Cells. J. Biochem. 136, 371-376.

    13. Tsai, HF, Liu, CS, Leu, TM, Wen, FC, Lin, SJ, Liu, CC, Yang, DK, Li, C, Hsieh M. (2004) An aly sis of trinucleotide repeats in different SCA loci in spinocerebellar ataxia patients and in nor ma l population of Taiwan. Acta Neurol Scand. 109:355-60.

    14. Tsai, HF, Liu, CS, Chen, GD, Lin, ML, Li, C, Chen YY, Wang BT and Hsieh M. (2003) Prenatal diagnosis of Machado-Joseph disease/ Spinocerebellar Ataxia Type 3 in Taiwan: early detection of expanded ataxin-3. Journal of Clinical Laboratory An aly sis.17:195-200.

    15. Wen, FC, Li, YH, Tsai, HF, Lin, CH, Li, C, Liu, SS, Lii, CK, Nukina, N, and Hsieh, M. (2003) Down-Regulation of Heat Shock Protein 27 in Neuronal Cells and Non-neuronal cells Expressing Mutant Ataxin-3. FEBS Letters. 546:307-14.

    16. Huang, H.-M., Tam, M. F., Tam, T-C S, Chen, D-H, Hsieh, M,. and Li, C. (2002) Proteomic an aly sis of stable protein methylation in lymphoblastoid cells. J. Biochem. 132: 813-818. (SCI)

    17. Lin, C-H, Huang, H.-M., Hsieh, M,. and Li, C. (2002) Arginine methylation of recombinant murine fibrillarin by protein arginine methyltransferase. J. Prot. Chem. 21:443-445. (SCI)
  2. 研討會
    • Li, C. Expression and function of prmt1 gene in zebrafish early development (2009) The 4th Asia-Oceania zebrafish meeting. August 31-September2, 2009, Jeju,

    • Tsai, Y.-R. , and Li, C. (2008) Effects of Prmt1 Knock-down on the Zebrafish Embryogenesis. Poster presentation in 20th Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) October 23-25, 2008, Taipei, Taiwan.

    • Li, C, Hu, H, Lee, Y, Li, Y, Chen, D, and Chang, H. (2008) Protein arginine methylation of the cellular nucleic acid binding protein (CNBP). Poster presentation in Joint 4th Asian and Oceania Hu ma n Proteome Organisation Congress (AOHUPO) and 2nd Pacific Rim International Conference on Protein Sciences (PRICPS) June 22-26, 2008, Cairns, QLD, Australia.

    • Hsieh, W.-Y., Lee, Y.-J., and Li, C. (2008) Study of Arginine Methylation of a RNA Binding Protein PAIRBP1. Poster presentation in the 23th Joint Annual Conference of Biomedical Science”

    • Li, C, Kap, E, and Simpson, R. (2007) Mass Spectrometric Identification of Arginine Methylation Sites in the Nuclear Matrix-Associated hnRNP Proteins. Poster presentation in Taiwan Proteomics Society International Conference 2007 (TPSIC2007) December, 4-5, 2007 NCKU, Tainan,

    • Hu, H-H, Chang H-H, Li, Y-J, Tsay, G J, Li, C (2007) Arginine Methylation of ZNF9 and putative differential recognition by anti-Sm autosera from SLE patients due to its methylation status.

    • Hou, P-T, Hung, C-M, Li, Y-J, and Li, C (2007) Developmental expression and functional an aly sis of PRMT1 gene in zebrafish. Oral presentation in the 22th Joint Annual Conference of Biomedical Science”.

    • Chang H-H, Hsu, T-C, Tsay, G J, and Li, C Differential recognition of proteins due to methylation status by anti-Sm autoantibodies from SLE patients. Poster presentation in the 13th Symposium on Recent Advances in Cellular and Molecular Biology”.

    • Hung, C.-J., and Li, C. (2005)Proteomic an aly ses of basic mouse brain proteins by chro ma tofocusing after nuclease treatment Poster presentation in the 13th Symposium on Recent Advances in Cellular and Molecular Biology”.

    • Li, C, Chen, D-H, and Hung, CJ. (2005) Modulation of type I protein arginine methyltransferase activity and protein methylation pattern by high ionic strength. “Poster presentation in the Experimental Biology 2005 Annual Meeting in San Diego, CA,

    • Wu, T.-T., Tsai, W.-T., Chu T.-T. and Li, C. (2004) An aly ses of the RET and EDNRB genes in patients with Hirschsprung disease in ” Oral presentation in 37th annual meeting of the Pacific Association of Pediatric Surgeons, Seoul, .

    • Hung C.-M., Wu T-T., Chen, K.-C., Lin C. Y. and Li, C. (2002) Mutation an aly ses of the RET gene in Hirschsprung disease in . “Poster presentation in the 10th SCBA International Symposium”

    • Hung, C.-J., Chen, T.-H., Lin, Y.-W. and Li, C. (2002) Characterization of protein arginine methyltransferase in porcine brain. “Poster presentation in the 10th SCBA International Symposium”

    • Chen, T. H. and Li, C. (2002) Subcellular localization of arginine methyl-accepting proteins in AdOX treated and nor ma l cells. “Poster presentation in the 10th SCBA International Symposium”

    • Huang, H.-M. and Li, C. (2001) Proteomic an aly sis of protein methylation in lymphoblastoid cells. “Poster presentation in the 9th SCBA International Symposium”

    • Huang, H.-M. and Li, C. (2001) Proteomic an aly sis of protein methylation in lymphoblastoid cells. Poster presentation in “Ninth Symposium on Recent Advances in Cellular and Molecular Biology”.
  3. 專書
    • Li, C. (1993) The physiological functions of the L-isoaspartyl protein methyltransferase and related proteins in bacteria. Ph. D. dissertation, Department of Chemistry and biochemistry, University of California , Los Angeles.
  4. 其他
    • 李娟 (2000)長生不老 蟲蟲有道 科學月刊, 363 219-221.
    • 李娟 (1997) (CAG)n重復增生疾病--中年發病的遺傳惡夢 科學月刊, 329