Interests in Research
My laboratory has been studying stable protein methylation since 1997 for mostly arginine methylation in lymphoblastoid cells and HeLa cells. We successfully utilized bioinformatic as well as proteomic tools to globally study protein arginine methylation both for the protein arginine methyltransferases (PRMT) and the methylaccepting proteins. Besides, we have been involved in studies of human genetic diseases such as fragile X mental retardation, spinocerebellar ataxia, dentatorubral and pallidoluysian atrophy and Hirschsprung disease.
We also use zebrafish as a model system to study protein arginine methylation We have successfully established the zebrafish culture system and analyzed the expression patterns of the zebrafish PRMT genes and knock-downed the expression of PRMT1 gene by antisense morpholino oligonucleotide (AMO). We have characterized the phenotypes of the morphants and are analyzing the arginine-methylated proteins in embryogenesis.
In our previous proteomic studies, we have identified a few RNA binding proteins that are candidate protein arginine methylated protein. We have obtained evidences of the modification and would like to further pursue the physiological roles of the modification. We thus would like to use cultured human cells as well as zebrafish as the model systems to characterize protein arginine methylation of specific RNA binding proteins and to investigate the involvement of the posttranslational modification in cellular function and embryogenesis.
Publications (at most 7 publications in recent 5 years)
- 51. Chang PH, Tsai YJ, Lee YJ, Pan H, and Li C,Expressional analysis of zebrafish serbp1a and serpine1 Chung Shan Medical Journal 2023; : -.(其他)
- Wang YC, Huang SH, Chang CP, Lai YC, Chan CH, Ou SC, Wang SH, Li C. and Li C,The conservation and diversity of the exons encoding the glycine and arginine rich domain of the fibrillarin gene in vertebrates, with special focus on reptiles and birds GENE 2023; 866: １-９.(SCI)
- Wang YC, Huang SH, Chang CP, and Li C,Identification and Characterization of Glycine- and Argi-nine-Rich Motifs in Proteins by a Novel GAR Motif Finder Program GENES 2023; 14: 1-20.(SCI)
- Yi-Chun Wang, Chien-Ping Changa, Yun-Jung Tsaia, Yu-Jen Leea, Chuan Li,Alternative 3′ splice site selection of intron 5 within the prmt8 gene results in a novel variant widely distributed in vertebrates and specifically abundant in Aves GENE 2020; 747: 1446-.(SCI)
- Yu-Jen Lee, Wen-Wei Chang, Chien-Ping Chang, Tsung-Yun Liu, Chun-Yi Chuang,Kun Qian, Y. George Zheng, and Chuan Li,Downregulation of PRMT1 promotes the senescence and migration of a non-MYCN amplifed neuroblastoma SK-N-SH cells Scientific Reports 2019; 9:1771: 1-14.(SCI)
- Yi-Chun Wang1, Chien-Wen Wang1, Wen-Chang Lin, Yun-Jung Tsai1, Chien-Ping Chang, Yu-Jen Lee, Min-Jon Lin, Chuan Li,Identification, chromosomal arrangements and expression analyses of the evolutionarily conserved prmt1 gene in chicken in comparison with its vertebrate paralogue prmt8. PLOS ONE 2017; 12(9): 0185-0185.(SCI)
- Chun-Yi Chuang1,2, Chien-Ping Chang3, Yu-Jen Lee3, Wei-long Lin1, Wen-Wei Chang3,4, Jia-Sian Wu3, Ya-Wen Cheng5 , Huei Lee5, and Chuan Li,PRMT1 expression is elevated in head and neck cancer and inhibition of protein arginine methylation by adenosine dialdehyde or PRMT1 knockdown down-regulate proliferation and migration of oral cancer cells Oncology Reports 2017; 38: 1115-1123.(SCI)
- Yi-Chun Wang, Li-Ching Chuang, Yu-Jen Lee, Chien-Ping Chang and Chuan Li,Analyses of gene structures and splicing variants of prmt1 and its vertebrate paralogue prmt8 suggest their molecular evolution Chung Shan Medical Journal 2016; 27: 39-50.(其他)
- Wang, YC, Wang, JD, Chen, CH, Chen, YW, and Li, C,A novel BLAST-Based Relative Distance (BBRD) method can effectively group members of protein arginine methyltransferases and suggest their evolutionary relationship Molecular Phylogenetics and Evolution 2015; 84: 101-111.(SCI)