陳威仁 副教授

Dr. Wei-Jen Chen

學歷:

• 博士：台灣大學醫學院生化暨分子生物學研究所，2000 ~ 2004
• 碩士：私立長庚醫學暨工程學院基礎醫學研究所，1995 ~ 1997
• 學士：國立陽明大學醫事技術學系檢驗組，1991 ~ 1995

經歷:

1. 中山醫學大學 生物醫學科學學系 副教授 2010.8 ~迄今
2. 中山醫學大學生命科學系助理教授，2005.8 ~2010.7
3. 台灣大學醫學院生化暨分子生物學研究所博士後研究員，2004.8 ~ 2005.7
4. 國軍八Ο三總醫院（國軍台中總醫院）醫檢科醫檢師，1998.7 ~ 1999.6

專長及教授課程:

• 分子生物學
• 生醫生化
• 分子生物學實驗
• 基因工程
• 腫瘤生物學

研究方向及計畫:

• 本實驗室研究方向著重於致力於天然化合物誘發癌症細胞凋亡和抑制癌症細胞生長、移行與轉移等防癌或抗癌機轉之研究。近期研究重心著眼於天然化合物白藜蘆醇 (resveratrol) 之結構類似物對抗乳癌細胞增生、轉移與上皮細胞間質細胞轉換機轉之探討。發現白藜蘆醇結構衍生物如3,5,3,4,5’-pentamethoxystilbene 能夠抑制乳癌細胞株 MCF-7細胞週期之運轉；另外也發現白藜蘆醇結構類似物3,5,4’-trimethoxystilbene 能抑制乳癌細胞株 MCF-7之上皮細胞間質細胞轉換並降低其侵襲力。這些研究提供以白藜蘆醇之結構為乳癌新藥開發先導藥物設計之基礎。

過去與目前正在進行研究計畫:

• 107年度科技部計畫: 探討EGCG是否藉由調節Wnt/β-catenin和PI3K/Akt訊號路徑來影響非小細胞肺癌中由TGF-β1所誘導的EMT轉換與侵襲性表型 (多年期)
• 106年度科技部計畫:Trans 3,5,4'-trimethoxystilbene 增加雌激素抗性乳癌細胞對tamoxifen感受性之分子機制研究
• 103~105年度科技部計畫:槲皮素 (quercetin) 抑制三陰性乳癌細胞上皮細胞-間質細胞轉換與侵襲性表型之分子機制研究 (多年期)
• 100~102年度科技部計畫:白藜蘆醇 (resveratrol) 與其甲氧基化結構衍生物抑制癌細胞上皮細胞-間質細胞轉換與侵襲性之分子機制研究  (多年期)
• 97~99年度科技部計畫:紫檀氏(pterostilbene)抗發炎及抗腫瘤增生作用機制之探討 (多年期)
• 96年度科技部計畫：以化學性蛋白質體學方法分析EGCG結合蛋白並評估其在化學癌症預防之重要性
• 95年度科技部計畫：以化學蛋白質體學方法分析茶紅素對蛋白酵素體抑制作用之選擇性
• 94年度科技部計畫：兒茶素EGCG抑制腫瘤相關性脂肪酸合成酵素機制之研究 

期刊論文(近五年代表著作):

• Kuei-Yang Han, Pei-Ni Chen, Ming-Chian Hong, You-Cheng Hseu, Ke-Ming Chen, Li-Sung Hsu, Wei-Jen Chen* (2018). Naringenin Attenuated Prostate Cancer Invasion via Reversal of Epithelial-to-Mesenchymal Transition and Inhibited uPA Activity. Anticancer Research. Vol. 38, pp. 6753-6758.
• Ddy Konelius, Hsin-Hua Li, Chiung-Huei Peng, Wei-Jen Chen, Yan-Zin Chang, Yi-Chao Bai, Stanley Liu, Chein-Ning Huang, and Chih-Li Lin* (2018). Liraglutide protects against glucolipotoxicity-induced RIN-m5F β-cell apoptosis through restoration of PDX1 expression. Journal of Cellular and Molecular Medicine. Vol. 23, pp. 619-629. doi: 10.1111/jcmm.13967.
• Ching-Chi Chang, Tzu-Chin Lin, Hsiao-Li Ho, Chien-Yin Kuo, Hsin-Hua Li, Tatiana A. Korolenko, Wei-Jen Chen, Te-Jen Lai, Ying-Jui Ho*, and Chih-Li Lin* (2018). GLP-1 Analogue Liraglutide Attenuates Mutant Huntingtin-Induced Neurotoxicity by Restoration of Neuronal Insulin Signaling. International Journal of Molecular Sciences. Vol. 19, pp. 2505-17. doi:10.3390/ijms19092505.
• Jie-Heng Tsai, Li-Sung Hsu, Hsiu-Chen Huang, Chih-Li Lin, Min-Hsiung Pan, Hui-Mei Hong, and Wei-Jen Chen* (2016). 1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione induces G1 cell cycle arrest and autophagy in HeLa cervical cancer cells. International Journal of Molecular Sciences. Vol. 17, pp. 1274-88. doi:10.3390/ijms17081274.
• James Cheng-Chung Wei, Hsiu-Chen Huang, Wei-Jen Chen, Chien-Ning Huang, Chiung-Huei Peng, and Chih-Li Lin* (2016). Epigallocatechin gallate attenuates amyloid β-induced inflammation and neurotoxicity in EOC 13.31 microglia. European Journal of Pharmacology. Vol. 770, pp. 16-24.
• Wai-Fai Tung, Wei-Jen Chen, Hui-Chih Hung, Guang-Yaw Liu, Jai-Nien Tung, Chien-Chih Huang, and Chih-Li Lin* (2015). 4-Phenylbutyric Acid (4-PBA) and Lithium cooperatively attenuate cell death during oxygen-glucose deprivation (OGD) and reoxygenation. Cellular and Molecular Neurobiology. Vol. 35, pp. 849-859.
• Cheng Huang, Yi Jing Chen, Wei-Jen Chen, Chih-Li Lin, Yu Xuan Wei, and Hsiu Chen Huang* (2015). Combined treatment with Chrysin and 1,2,3,4,6-Penta-O-Galloyl-β-D-Glucose synergistically inhibits LRP6 and Skp2 activation in triple-negative breast cancer and xenografts. Molecular Carcinogenesis. Vol. 54, pp. 1613-1625.

研討會議論文:

• Wei, C. S. and Chen, W. J.* (2009). Effects of pterostilbene on heregulin-β1-associated signal transduction of hu ma n breast cancer cells. 第二十四屆生物醫學聯合學術年會.
• Tsai, J. H. and Chen, W. J.* (2009). Studies on the molecular mechanisms of 3,5,3',4',5'-pentamethoxystilbene induced G1 cell cycle arrest in MCF-7 hu ma n breast cancer cells. 第二十四屆生物醫學聯合學術年會.
• Lin, Y. T. and Chen, W. J.* (2008). Suppression of inducible nitric oxide synthase in lipopolysaccharides-activated ma crophages by pterostilbene. Sixteen symposium on recent advances in cellular and molecular biology. 第16屆細胞及分子生物新知研討會.
• Lin, C. C. and Chen, W. J.* (2007). Study on the mechanisms by which EGCG inhibits heregulin-β1-induced Fas expression. 第二十二屆生物醫學聯合學術年會.
• Chen, W. J.* (2006). Mechanisms by which (-)-epigallocatechin 3-gallate inhibits heregulin-beta1-induced fatty acid synthase expression in breast cancer cell lines. 232nd ACS National Meeting & Exposition.
• Lin, J. K., Chen, W. J. and Lin-Shiau, S. Y. (2005). Pentagalloylglucose induces G1 arrest and apoptosis through suppressing proteasome activity and el eva ting Cdk inhibitors and Bax proteins in hu ma n Jurkat T cells. Am eric an Association for Cancer research, 96th Annual Meeting.

專書:

• Pan, M. H. and Chen, W. J.* (2008) ACS Books "Functional Food and Health.": The cancer preventive potential of tea polyphenol EGCG in HER2-positive breast cancer. 2008 pp. 335-344
• Pan, M. H., Chen, W. J., Lo, C. H., Li, S., Sang, S., and Ho, C. H. (2008) ACS Books "Functional Food and Health.": Induction of apoptosis by acetylated black tea polyphenol through reactive oxygen species production, cytochrome c release, and caspase activation in hu ma n leukemia HL-60 cells. 2008 pp. 345-361